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KMID : 0882419930440050613
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Korean Journal of Medicine
1993 Volume.44 No. 5 p.613 ~ p.619
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Infection of Cultured Human Umbilical Vein Endothelial Cells with Hantaan Virus
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유석희
강응택/정상인/양용태/이병직
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Abstract
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ackground : The pathogenesis of korean hemorrhagic fever (Hemorrhagic fever with renal
syndrome) is not elucidated until today. The basic mechaism of disease is vascular injury
which increases its permeability. This study took aim to demonstrate the tropism of Hataan
virus to human umbilical vein endothelial cells (HUVEC) and the infectivity of virus
proliferated in HUVEC.
Methods : Cultured HUVEC were inoculated with Hantaan virus (strain 76-118).
Replication of Hantaan virus in HUVEC was demonstrated by immunofluorescence and
immunohisto chemistry using mouse anti-Hantaan virus monclonal antibody. The infectivity
of replicated virus in was determined with Vero E-6 cells.
Results ; The infectivity of Hantaan virus was 10E4. After 3 days after virus inoculation,
specific fine granulr fluorecsence was found in the cytoplasm of HUVEC. After 7 days
nearly all of cells showed specific fluorescence. Infected HUVEC could infect Vero E-6 cells.
Conclusions ; We demonstrated the tropism of Hantaan virus to cultured HUVEC and
infectivity of virus in infected HUVEC.
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KEYWORD
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